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Regulation of the adenylyl cyclase signaling system in various types of cultured endothelial cells
Author(s) -
Manolopoulos Vangelis G.,
Samet Mark M.,
Lelkes Peter I.
Publication year - 1995
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.240570403
Subject(s) - adenylyl cyclase , forskolin , adcy9 , adcy10 , medicine , endocrinology , ibmx , camp dependent pathway , gs alpha subunit , phosphodiesterase , endothelial stem cell , biology , microbiology and biotechnology , receptor , umbilical vein , stimulation , chemistry , biochemistry , enzyme , in vitro
We studied the effects of modulators of the adenylyl cyclase pathway on the accumulation of cAMP in endothelial cells isolated from bovine aortas, pig pulmonary arteries, human umbilical veins, and human subcutaneous adipose microvessels. In addition to quantitative differences in the basal levels, cAMP stimulation in different endothelial cell types varied in sensitivity and magnitude in response to both the direct adenylyl cyclase activator forskolin and the β‐adrenergic receptor agonist isoproterenol. Furthermore, the ubiquitous phosphodiesterase inhibitor IBMX differentially enhanced both the basal and the stimulated cAMP levels in the various cell types. Histamine caused an elevation of cAMP only in bovine aortic endothelial cells and in human umbilical vein endothelial cells. Treatment of the cells with cholera and pertussis toxins, which uniquely affect G‐protein subunits, resulted in divergent elevation of cAMP in the various cells. Thus, in each cell type, a distinct profile of regulation of the cAMP levels was found. Our results suggest that the adenylyl cyclase signaling system in various types of endothelial cells can be differentially regulated at the levels of receptors, G‐proteins, adenylyl cyclase, and phosphodiesterase.