Premium
Enhanced expression of calcium‐binding protein regucalcin mRNA in regenerating rat liver
Author(s) -
Yamaguchi Masayoshi,
Kanayama Yoshitaka
Publication year - 1995
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.240570203
Subject(s) - messenger rna , intraperitoneal injection , endocrinology , medicine , calmodulin , calcium , calcium binding protein , trifluoperazine , biology , northern blot , hepatectomy , liver regeneration , gene expression , gene , biochemistry , regeneration (biology) , surgery , resection , microbiology and biotechnology
The expression of hepatic calcium‐binding protein regucalcin mRNA was investigated in regenerating rat liver. The change of regucalcin mRNA levels was analyzed by Northern blotting, using liver regucalcin cDNA (0.9 kb with complete open reading frame). The reduced liver weight by partial hepatectomy (about 70%) was completely restored at 3 days after surgery. Regenerating liver significantly increased calcium content. Liver regucalcin mRNA levels clearly increased 1–5 days after hepatectomy, in comparison with that of sham‐operated rats, although the increase was not seen 12 hr after the surgery. Increased regucalcin mRNA levels in regenerating liver were appreciably reduced by single intraperitoneal administration of actinomycin D (100 μg/100 g body weight), an inhibitor of transcriptional process. Moreover, the increased regucalcin mRNA levels by hepatectomy was weakened by a single intraperitoneal administration of trifluoperazine (2.5 mg/100 g), an inhibitor of Ca 2+ /calmodulin. These findings demonstrate that the expression of hepatic regucalcin mRNA is enhanced in regenerating rat liver.