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Conformational information in DNA: Its role in the interaction with DNA topoisomerase I and nucleosomes
Author(s) -
Caserta Micaela,
Venditti Patrizia,
Mauro Ernesto Di,
Camilloni Giorgio,
Venditti Sabrina
Publication year - 1994
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.240550111
Subject(s) - dna supercoil , topoisomerase , dna , nucleosome , dna clamp , transcription (linguistics) , hmg box , biology , computational biology , microbiology and biotechnology , biophysics , genetics , chemistry , transcription factor , chromatin , rna , gene , dna binding protein , dna replication , reverse transcriptase , linguistics , philosophy
Information in DNA is not limited to sequence information. Both local and global conformational parameters are pivotal to the interaction with a number of relevant proteins. The function of the major components of the transcription machinery (RNA polymerase II, DNA topoisomerase I, nucleosomes, the TATA‐binding factor) is dependent on the topological status of the substrate DNA molecule. The topological requirements and the conformational consensus that dictate the rules for localization of nucleosomes and define the active sites for DNA topoisomerase I have been established; the reaction of DNA topoisomerase I is regulated by a topological feedback mechanism. The integrating function of the free energy of supercoiling in the transcription process and the regulatory role of DNA topoisomerase I are discussed. © 1994 Wiley‐Liss, Inc.