Premium
Subnuclear distribution of the vitamin D receptor
Author(s) -
Bidwell Joseph P.,
van Wijnen André J.,
Fey Edward G.,
Merriman Harold,
Penman Sheldon,
Stein Janet L.,
Stein Gary S.,
Lian Jane B.
Publication year - 1994
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.240540417
Subject(s) - nuclear matrix , calcitriol receptor , nuclear receptor , biology , microbiology and biotechnology , nuclear receptor co repressor 1 , osteocalcin , nuclear protein , transcription factor , receptor , gene , biochemistry , chromatin , alkaline phosphatase , enzyme
The subnuclear distribution of the vitamin D receptor was investigated to begin addressing the contribution of nuclear architecture to vitamin D–responsive control of gene expression in ROS 17/2.8 rat osteosarcoma cells. The nuclear matrix is an anastomosing network of filaments that is functionally associated with DNA replication, transcription, and RNA processing. The representation of vitamin D receptor in the nuclear matrix and nonmatrix nuclear fractions was determined by the combined application of (1) sequence‐specific interactions with the vitamin D receptor binding element of the rat bone‐specific osteocalcin gene promoter and (2) Western blot analysis. Both methods confirmed the presence of vitamin D receptor in the nonmatrix nuclear fraction and the absence of detectable vitamin D receptors associated with the nuclear matrix. In contrast, these same nuclear matrix proteins preparations exhibited association with the general transcription factor AP‐1 and a bone tissue‐specific promoter binding factor NMP2. NMP‐2 exhibits recognition for a promoter domain contiguous to the vitamin D‐responsive element of the osteocalcin gene, although the vitamin D receptor does not appear to be a component of the nuclear matrix proteins. Interrelationships between nuclear matrix proteins and nonmatrix nuclear proteins, in mediating steroid hormone responsiveness of a vitamin D–regulated promoter, are therefore suggested.