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Specific region of the c‐ myc promoter is responsive to electric and magnetic fields
Author(s) -
Lin Hana,
Goodman Reba,
ShirleyHenderson Ann
Publication year - 1994
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.240540304
Subject(s) - transfection , gene , dna , microbiology and biotechnology , upstream (networking) , promoter , biology , transcription (linguistics) , gene expression , genetics , computer network , computer science , linguistics , philosophy
The level of c‐ myc transcripts is increased in cells exposed to extremely low frequency (elf) electromagnetic (EM) fields at 60 Hz. The aim of the present experiments was to determine if regulatory regions upstream of the c‐ myc gene modulate the response to EM fields. DNA upstream of P1 of both mouse and human c‐ myc genes was transfected into cells as CAT constructs. The presence of DNA 5′ to the human or mouse myc genes results in increased expression of CAT following 20 min exposures of cells to 60 Hz elf EM fields. Specific portions of the human upstream DNA were deleted and introduced into cells. The region responsive to EM fields is located between −353 and −1,257 relative to the P 1 promoter.