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Differential expression of the two VEGF receptors flt and KDR in placenta and vascular endothelial cells
Author(s) -
Barleon Bernhard,
Hauser Stefanie,
Schöllmann Claudia,
Weindel Karin,
Marmé Dieter,
Yayon Avner,
Weich Herbert A.
Publication year - 1994
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.240540107
Subject(s) - vascular endothelial growth factor b , vascular endothelial growth factor , vascular endothelial growth factor c , kinase insert domain receptor , vascular endothelial growth factor a , biology , receptor tyrosine kinase , vascular endothelial growth inhibitor , microbiology and biotechnology , receptor , s1pr1 , tyrosine kinase , placenta , cancer research , signal transduction , vegf receptors , biochemistry , fetus , genetics , pregnancy
Vascular endothelial growth factor (VEGF) is a newly identified growth and permeability factor with a unique specificity for endothelial cells. Recently the flt‐encoded tyrosine kinase was characterized as a receptor for VEGF. A novel tyrosine kinase receptor encoded by the KDR gene was also found to bind VEGF with high affinity when expressed in CMT‐3 cells. Screening for flt and KDR expression in a variety of species and tissue‐derived endothelial cells demonstrates that flt is predominantly expressed in human placenta and human vascular endothelial cells. Placenta growth factor (PIGF), a growth factor significantly related to VEGF, is coexpressed with flt in placenta and human vascular endothelial cells. KDR is more widely distributed and expressed in all vessel‐derived endothelial cells. These data demonstrate that cultured human endothelial cells isolated from different tissues express both VEGF receptors in relative high levels and, additionally, that all investigated nonhuman endothelial cells in culture are also positive for KDR gene expression.