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Catecholamine stimulated lipolysis in differentiated human preadipocytes in a serum‐free, defined medium
Author(s) -
van de Venter Maryna,
Litthauer Derek,
Oelofsen Willem
Publication year - 1994
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.240540102
Subject(s) - lipolysis , medicine , endocrinology , adipose tissue , adipocyte , catecholamine , adrenergic receptor , chemistry , adrenergic , stromal vascular fraction , lipid droplet , receptor , 3t3 l1 , biology
Adipocyte precursors from the stromal vascular fraction of human adipose tissue were allowed to differentiate in serum‐free defined medium, whereafter their catecholamine stimulated lipolytic response was compared to that of mature isolated human adipocytes. Seventy‐five to ninety percent of the fibroblast‐like cells accumulated lipid droplets and glycerol‐3‐phosphate dehydrogenase activities of 1,000–2,800 mU/mg protein were measured in cell homogenates of differentiated cells. Lipolysis could be stimulated by both isoproterenol and norepinephrine in both differentiated preadipocytes as well as mature adipocytes. The results obtained with β‐adrenergic agents suggested the presence of a higher affinity receptor in differentiated preadipocytes as compared to mature adipocytes. Mature adipocytes responded well to β‐adrenergic agents, but no antilipolytic α 2 ‐adrenergic response was observed in the differentiated preadipocytes. The presence of Gi proteins in the differentiated preadipocytes was suggested by the antilipolytic effect of adenosine as well as the lipolytic activity generated by pertussis toxin. In conclusion, our medium supported the differentiation of a very high percentage of human preadipocytes which developed a sensitive β‐adrenergic lipolytic response but which lacked an α 2 ‐adrenergic antilipolytic response.