Premium
Quantitation of estrogen and progesterone receptors by immunocytochemical and image analyses
Author(s) -
Aziz Douglas C.,
Barathur Raj R.
Publication year - 1993
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.240531145
Subject(s) - estrogen receptor , estrogen , progesterone receptor , immunoassay , receptor , concordance , histogram , biology , pathology , medicine , endocrinology , breast cancer , antibody , artificial intelligence , image (mathematics) , computer science , cancer , immunology
The ability to detect estrogen and progesterone receptors by immunocytochemical analysis in formalin‐fixed, paraffin‐embedded sections has clear advantages over other techniques, including the ability to assay small biopsy specimens, fine needle aspirate samples, and archival material. Twenty‐two cases of breast carcinoma were evaluated for estrogen and progesterone receptors by immunocytochemical analysis and enzyme immunoassay. Using a true color‐based image analysis system, histograms of area versus the optical density of the positive staining nuclei were generated. A binary decision algorithm was derived from these histogram parameters by the Classification and Regression Trees (CART) computer program. Estimates generated by the algorithm for image analysis/immunocyto‐chemical analysis had a 90% concordance with the enzyme immunoassay values. We conclude that quantitative immunocytochemical results for estrogen and progesterone receptor content in formalin‐fixed, paraffin‐embedded tissue can be generated using image analysis.