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Nitric oxide production from a macrophage cell line: Interaction with autologous and allogeneic lymphocytes
Author(s) -
Isobe KenIchi,
Nakashima Izumi
Publication year - 1993
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.240530304
Subject(s) - nitric oxide , nitrite , macrophage , microbiology and biotechnology , stimulation , cell culture , biology , exudate , spleen , chemistry , immunology , biochemistry , endocrinology , in vitro , nitrate , ecology , genetics , botany
The indirect stimulation of macrophages to produce nitrite was examined by using the macrophage cell line J774. J774 spontaneously produced nitrite, when cultured at high concentration. J774 cultured in low concentration ( < 10 4 cells in 100 μl) barely produced nitrite. J774 cultured in low concentration produced a large amount of nitrite by the co‐culture of nonadherent spleen cells or nonadherent peritoneal exudate cells, which were stimulated with con A, anti‐CD3, or staphylococcal enterotoxin A. J774 (BALB/c derived: H‐2 d ) cultured with either syngeneic (BALB/c) or allogeneic (B6; H‐2 b B10BR; H‐2 k ) nonadherent lymphocytes, which were stimulated with conA or anti‐CD3, produced nitric oxide. However, J774 produced nitric oxide by stimulation with SEA only when co‐cultured with SEA‐reactive T lymphocytes. Peritoneal exudate cells from mice, which did not proliferate by the stimulation of conA or anti‐CD3, proliferated well by the addition of L‐arginine homologue, N G ‐monomethyl‐L‐arginine. The proliferation of nonadherent peritoneal exudate cells stimulated with conA or anti‐CD3 was suppressed by the addition of peritoneal macrophages. This suppression was abolished by the addition of N G ‐monomethyl‐L‐arginine.