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Unambiguous identification of histone H 1 in Trypanosoma cruzi
Author(s) -
Toro G. Cecilia,
Galanti Norbel,
Hellman Ulf,
Wernstedt Christer
Publication year - 1993
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.240520407
Subject(s) - edman degradation , histone h1 , histone , chromatin , biochemistry , biology , proteases , trypanosoma cruzi , microbiology and biotechnology , peptide sequence , chemistry , dna , gene , enzyme , parasite hosting , world wide web , computer science
The existence of histone H 1 has been questioned in Trypanosomatids. We report here the presence of a histone H 1 in the chromatin of Trypanosoma cruzi . This protein was purified by narrow‐bore reversed phase HPLC and its amino acid composition analyzed and compared with histones H 1 from other species. Furthermore, the purified chromosomal protein was digested with proteases and the amino acid sequences of the resulting peptides were analyzed by the automated Edman degradation. The sequences obtained were found to present a high degree of homology when compared to the carboxy terminal domain of other known histones H 1 .