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ADP‐ribosylation of nucleolar proteins in HeLa tumor cells
Author(s) -
Leitinger Norbert,
WesierskaGadek Jozefa
Publication year - 1993
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.240520207
Subject(s) - nucleolus , nucleolin , hela , adp ribosylation , microbiology and biotechnology , chemistry , electrophoresis , polyacrylamide gel electrophoresis , biochemistry , nuclear protein , biology , nad+ kinase , cytoplasm , cell , enzyme , gene , transcription factor
ADP‐ribosylation reactions in nucleoli of exponentially growing HeLa cells were studied. Isolated nuclei or nucleoli were labeled with 32 P‐NAD; then the nucleolar proteins were analyzed by 1‐dimensional and 2‐dimensional polyacrylamide gel electrophoresis (PAGE) and modified proteins were detected by autoradiography. The labeled nucleolar proteins were also chromatographically fractionated on DEAE‐cellulose. Electrophoretic analysis of total nucleolar and chromatographically purified proteins revealed that besides nuclear ADP‐ribosyltransferase and histones two characteristic nucleolar phosphoproteins numatrin/B23 and nucleolin/C23 were modified by ADP‐ribosylation.