Premium
Differential expression of the histone H1° gene in U937 and HL‐60 leukemia cell lines
Author(s) -
Hochhuth C.,
Doenecke D.
Publication year - 1992
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.240500312
Subject(s) - cell culture , u937 cell , microbiology and biotechnology , gene expression , messenger rna , gene , histone , biology , butyrate , leukemia , chemistry , genetics , biochemistry , fermentation
The expression of the human H1° histone gene and of a main type H1 gene was analyzed in two human leukemia cell lines. The main type, replication dependent H1 gene expression reflected the state of proliferation of both cell lines. No H1° mRNA was detected in the promyelocytic HL‐60 line, whereas the monocytic U937 cells showed low steady‐state levels of H1° mRNA. Stimulation of HL‐60 with several known induces of differentiation failed to induce any accumulation of H1°. mRNA. Treatment of U937 with phorbol ester or butyrate, on the other hand, led to an increase of the H1° mRNA concentration. © 1992 Wiley‐Liss, Inc.