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Recombinant murine steel factor stimulates in vitro production of granulocyte–macrophage progenitor cells
Author(s) -
Williams Douglas E.,
Foxworthe Diane,
Teepe Mark,
Eisenman June,
Lyman Stewart D,
Anderson Dirk
Publication year - 1992
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.240500302
Subject(s) - granulocyte macrophage colony stimulating factor , progenitor cell , bone marrow , in vitro , granulocyte , recombinant dna , colony stimulating factor , macrophage , biology , population , cfu gm , microbiology and biotechnology , immunology , andrology , haematopoiesis , chemistry , stem cell , medicine , biochemistry , gene , environmental health
Abstract The ability of murine Steel factor to promote the in vitro production of granulocyte‐macrophage progenitor cells (CFU‐GM) was examined in short‐term liquid cultures. Bone marrow from C57BL/6J or Sl/Sl d mice was placed in culture for seven days with either Steel factor alone or in the presence of IL‐3. CFU‐GM responsive to GM‐CSF, IL‐3, and CSF‐1 were measured in the input population and again after 3 or 7 days in culture. Steel factor alone increased the number of all CUF‐GM types as early as 3 days after culture initiation, with further increases at day 7. This effect was protentiated by the addition of IL‐3. Production of CFU‐GM by C57BL/6J or Sl / Sl d marrow was comparable except for enhanced production of CSF‐1 responsive progenitors by Sl / Sl d marrow. A recombinant Sl d protein was also shown to be equivalent to the wild‐type protein in its capacity to promote CFU‐GM production from normal bone marrow. © 1992 Wiley‐Liss, Inc.