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Collagen increases the synthesis of membrane‐associated proteoglycans produced by sertoli cells
Author(s) -
Rodriguez J. Pablo,
Minguell Jose J.
Publication year - 1992
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.240500106
Subject(s) - sertoli cell , microbiology and biotechnology , chemistry , membrane , proteoglycan , cell , biochemistry , biophysics , extracellular matrix , biology , endocrinology , spermatogenesis
Sertoli cells in culture produce two isoforms of proteoglycans which are found in the culture medium and associated with the cell membrane. The amount of both types of proteoglycans increased when Sertoli cells were plated on type 1 collagen‐coated dishes as compared to uncoated dishes. The effect is due to an increase in the synthesis of proteoglycans rather than a diminished rate of degradation of these molecules. The collagen substrate also affects the distribution of these macromolecules; an increase in the amount of membrane‐associated proteoglycans occurs at the expense of the proteoglycans released to the culture medium. © 1992 Wiley‐Liss, Inc.