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The inhibition of ATP‐dependent shape change of human erythrocyte ghosts correlates with an inhibition of Mg 2+ ‐ATPase activity by fluoride and aluminofluoride complexes
Author(s) -
Morris Michael B.,
Monteith Gregory,
Roufogalis Basil D.
Publication year - 1992
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.240480404
Subject(s) - chemistry , fluoride , sodium fluoride , ouabain , atpase , vanadate , egta , aluminium , inorganic chemistry , nuclear chemistry , sodium , biochemistry , biophysics , enzyme , calcium , organic chemistry , biology
The vanadate‐sensitive Mg 2+ ‐dependent ATPase activity of the human erythrocyte ghost is believed to be involved in the shape change events that convert echinocytic ghosts to smoothed forms (biconcave discs and stomatocytes). At physiological salt concentration, pH 7.4, 2 mMATP, 5 mM Mg 2+ and 1 mM EGTA, the Mg 2+ ‐ATPase activity of ghosts was inhibited strongly by millimolar concentrations of sodium fluoride: I 50 = 1.31 ± 0.2, mM (mean ± S.D., n = 12). The addition of aluminium chloride to 15 μM reduced the concentration of NaF required for 50% inhibition to 0.76 ± 0.21 mM (n = 10). Aluminium alone had only a small inhibitory effect of the ATPase activity (13 ± 9 %; n = 10). Desferrioxamine, a strong chalator of tervalent aluminium ion, failed to reverse the inbibition by fluoride and reversed the inhibition in the presence of aluminium and fluoride back to those values obtained with fluoride alone. Of several metal salts tested only beryllium sulfate was able to replace aluminium as an effective inhibitor in the presence of fluoride. Inhibition of the Mg 2+ ‐ATPase activity by fluoride and the aluminofluoride complexes correlated with an inhibition of the rate of MgATP‐dependent change in red cell ghost shape from echinocytes to smoothed forms. All gross morphological changes of the smoothing process were affected, including the production of discocytes, stomatocytes and endocyctic vesicles.