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Butanol‐extractable and detergent‐solubilized cell surface components from murine large cell lymphoma cells associated with adhesion to organ microvessel endothelial cells
Author(s) -
Tressler Robert J.,
Nicolson Garth L.
Publication year - 1992
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.240480208
Subject(s) - microvessel , cell , chemistry , endothelial stem cell , cell adhesion , adhesion , butanol , solubilization , microbiology and biotechnology , biology , biochemistry , immunology , in vitro , immunohistochemistry , organic chemistry , ethanol
Abstract Metastatic variant cell lines of the murine RAW117 large cell lymphoma were used to study the cell surface components involved in syngeneic tumor cell/microvessel endothelial cell interactions. Poorly liver‐metastatic parental RAW 117‐P cell line adhered to murine hepatic sinusoidal endothelial cell monolayers at significantly lower rates than the liver‐selected, highly liver‐metastatic RAW117‐H10 line and both cell lines were poorly adherent to lung microvessel and bovine aorta endothelial cells. Viable, 2% 1‐butanol‐treated RAW117‐H10 tumor cells formed fewer liver tumor nodules in experimental metastasis assays than untreated H10 cells and were significantly less adherent to murine hepatic sinusoidal endothelial cell monolayers. When 2% 1‐butanol extracts of metabolically labeled or CHAPS detergent Iysates of cell surface‐labeled tumor cells were analyzed for their ability to bind to fixed microvessel endothelial cell monolayers, quantitative differences were found in the extractable tumor cell surface components that bound to the different organ‐derived microvessel endothelial cells. Cell surface components (1‐butanol extractable), of M r ∼ 85,000–90,000 and ∼ 37,000–40,000 bound to hepatic sinusoidal endothelial cell monolayers to a greater extent than to murine lung microvessel endothelial or bovine aortic endothelial cell monolayers, Whereas tumor cell surface components of M r ∼ 45,000, ∼ 33,000, and ∼ 25,000 bound similarly to endothelial cells regardless of origin. The results suggest but do not prove that tumor cell/endothelial cell adhesion involves multiple tumor cell surface components, some of which commonly bind to various endothelial cells and others for which binding may be dictated by the tissue origin and type of endothelial cell. Particular RAW117 butanol‐extractable cell membrane components were associated with tumor cell‐endothelial cell adhesion, and these components could be responsible, in part, for the preferential adhesion of RAW117 cells to liver sinusoidal endothelial cells and metastasis to liver.