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Translational control
Author(s) -
Laura L. Arthur,
Slavica,
Pavlovic-Djuranovic,
Kristin S. Koutmou
Publication year - 1991
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.240470805
Subject(s) - citation , computer science , control (management) , information retrieval , library science , world wide web , artificial intelligence
The ribosome has been a driver for single-particle cryo-EM methods development since the 1970s. We have a long-standing collaboration with the Korostelev lab (University of Massachusetts Medical School) on translational control of gene expression by the ribosome. In a recent example, we visualized the stepwise decoding of cognate aminoacyl-tRNA [1] [2]. Using the “focused mask” feature of Frealign, we were able to resolve small structural differences between three states of tRNA accommodation in the A site of the small subunit of a bacterial ribosome. The accommodation of cognate tRNA, and rejection of other tRNAs is a fundamental requirement for fidelity in ribosomal translation. Our work suggests that the 30S subunit only efficiently closes when a cognate tRNA is bound, moving the elongation factor EF-Tu into position to be activated for GTP hydrolysis by the sarcin-ricin loop.