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Binding of glucocorticoid receptors to model DNA response elements
Author(s) -
BenoreParsons Marilee,
Liebman Jeffrey,
Wennogle Lawrence P.
Publication year - 1991
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.240470407
Subject(s) - glucocorticoid receptor , glucocorticoid , receptor , pelp 1 , dna , microbiology and biotechnology , biology , chemistry , computational biology , genetics , endocrinology , nuclear receptor , transcription factor , gene
DNA sequences were synthesized that contained the consensus 15‐base pair glucocorticoid receptor binding site linked to flanking sequences of various lengths. Binding of these synthetic oligomers to glucocorticoid receptor, employing a reconstituted binding system with purified components, indicated that a minimal size of approximately 45 base pairs was necessary to bind the receptor optimally. Sequences containing multiple receptor binding sites competed more effectively for binding. These findings are consistent with recent demonstrations that multiple control elements act synergistically to affect transcriptional control by glucocorticoids and confirm that regions flanking the consensus GRE binding site are instrumental in optimizing binding interactions.