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Buffering of intracellular calcium in response to increased extracellular levels in mortal, immortal, and transformed human breast epithelial cells
Author(s) -
Ochieng Josiah,
Tahin Quivo S.,
Booth Charles C.,
Russo Jose
Publication year - 1991
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.240460308
Subject(s) - extracellular , intracellular , calcium in biology , microbiology and biotechnology , calcium , human breast , chemistry , biology , medicine , breast cancer , cancer
Extracellular levels of calcium at 1.05 mM or higher induce terminal differentiation and senescence in the mortal (MCF‐10M) line of human breast epithelial cells, but does not retard the growth or induce differentiation in the immortal (MCF‐10A) and oncogene transformed (MCF‐10AneoT) lines. Intracellular levels of calcium and inositol triphosphate were determined in MCF‐10M, MCF‐10A, and MCF‐10AneoT, under conditions of low and high extracellular calcium. We hereby report that increases in extracellular calcium is translated into significant increases in intracellular levels of calcium and inositol triphosphate in MCF‐10M, but not in MCF‐10A and MCF‐10AneoT. This difference in the apparent calcium buffering capacity between the mortal and the immortalized human breast epithelial cells could account for the latter's unperturbed growth potential in high extracellular calcium environment.