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Applications of immortalized cells in basic and clinical neurology
Author(s) -
Geller Herbert M.,
Quiñ;onesJenab Vanya,
Poltorak Maciej,
Freed William J.
Publication year - 1991
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.240450310
Subject(s) - immortalised cell line , cell culture , microbiology and biotechnology , biology , cell , gene , function (biology) , primary cell , cell growth , cancer research , neuroscience , genetics
Immortalized cell lines can serve as model systems for studies of neuronal development and restoration of function in models of neurological disease. Cell lines which result from spontaneous or experimentally‐induced tumors have been used for these purposes. More recently, the techniques of genetic engineering have resulted in the production of cell lines with specific desired characteristics. This has been accomplished by insertion of a desired gene into a pre‐existing immortal cell or by immortalizing primary cells. The production of immortal cell lines using temperature‐sensitive immortalizing genes offers an additional method of controlling gene expression, and thereby controlling cell proliferation and differentation. In the nervous system, these techniques have produced immortal cell lines with neuronal and glial properties.