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Biochemical changes associated with the mechanism controlling superoxide radical formation in the aging rotifer
Author(s) -
Sawada Masaaki,
Carlson John C.
Publication year - 1990
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.240440304
Subject(s) - superoxide dismutase , catalase , rotifer , chemistry , lipid peroxide , superoxide , phospholipase a2 , biochemistry , phospholipase , enzyme , biology , ecology
Levels of the superoxide radical (SOR) and lipid peroxides were measured and found to increase during aging in the short‐lived rotifer, Asplanchna brightwelli . Life‐span was altered by changes in environmental temperature, absence of light, diet restriction, exposure to ultraviolet radiation, and addition of vitamin E to the diet. Each of the conditions that lengthened life‐span decreased SOR and lipid peroxide levels, and each condition that shortened life‐span increased levels of SOR and lipid peroxides. Additional experiments indicated that on the third day of age, there was a significant increase in Ca 2+ uptake and phospholipase A 2 activity in membrane samples and an elevation in superoxide dismutase and catalase activity in rotifer homogenates. In addition, SOR concentration was inhibited by the addition of bromophenacyl bromide and indomethacin to membrane samples. By day 5 there was also a significant increase in the lysosomal enzyme, α‐mannosidase. The results of this study indicate that levels of the SOR and lipid peroxides are coupled to rotifer life‐span and that activation of phospholipase A 2 may contribute to the elevation of these agents in older animals.