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Clustering of sequential enzymes in the glycolytic pathway and the citric acid cycle
Author(s) -
Beeckmans Sonia,
Van Driessche Edilbert,
Kanarek Louis
Publication year - 1990
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.240430402
Subject(s) - glycolysis , citric acid cycle , cluster analysis , enzyme , citric acid , chemistry , biochemistry , computer science , artificial intelligence
In recent years, evidence has been accumulating that metabolic pathways are organized in vivo as multienzyme clusters. Affinity electrophoresis proves to be an attractive in vitro method to further evidence specific associations between purified consecutive enzymes from the glycolytic Pathway on the one hand, and from the citric acid cycle on the other hand. Our results support the hypothesis of cluster formation between the glycolytic enzymes aldolase, glyceraldehydephosphate dehy‐drogenase, and triosephosphate isomerase, and between the cycle enzymes fumarase, malate dehydrogenase, and citrate synthase. A model is presented to explain the possibility of regulation of the citric acid cycle by varying enzyme‐enzyme associations between the latter three enzymes, in response to changing local intramitochondrial ATP/ADP ratios.