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Retransformation of a revertant cell line with the adenovirus E1 oncogenes and vanadate
Author(s) -
Dessureault Jean,
Weber Joseph M.
Publication year - 1990
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.240430309
Subject(s) - reversion , plasmid , transfection , cell culture , biology , transformation (genetics) , gene , microbiology and biotechnology , mutation , phenotype , vanadate , genetics , biochemistry
We have isolated a revertant cell line (G5) from an adenovirus transformed rat cell line (F4) which failed to express the integrated viral oncogenes. To determine whether the reversion mutation was acting in cis or trans the G5 cells were co‐transfected with an E1 gene bearing expression plasmid and a neomycin photransferase bearing plasmid. 0418‐resistant colonies were picked and shown to express the E1 proteins and to be tumorigenic. This re‐transformation could be partially mimicked by treatment with vanadate, an inhibitor of phosphotyrosine phosphatases. These results show that the continued presence of the E1 proteins was required to maintain the transformed phenotype, and that the reversion mutation was a cis‐acting event affecting directly the integrated E1 genes.