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Lung carcinoid cell lines have Bombesin‐like peptides and EGF receptors
Author(s) -
Moody T. W.,
Lee M.,
Kris R. M.,
Bellot F.,
Bepler G.,
Oie H.,
Gazdar A. F.
Publication year - 1990
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.240430205
Subject(s) - bombesin , cell culture , receptor , biology , epidermal growth factor , cell , western blot , cancer research , microbiology and biotechnology , medicine , biochemistry , neuropeptide , gene , genetics
Abstract The biochemical properties of lung cancer cell lines were investigated. Bombesin‐like peptides were present in three small cell lung cancer (SCLC) cell lines examined and three of four lung carcinoids but not in five non‐small cell lung cancer (NSCLC) cell lines. Therefore SCLC and some lung carcinoids, but not NSCLC, are enriched in neuroendocrine properties. In contrast, 125 I‐EGF bound with high affinity to all five NSCLC cell lines and three of four lung carcinoids but not to the three SCLC cell lines examined. For lung carcinoid cell line NCI‐H727, 125 I‐EGF bound with high affinity (Kd = 6 nM) to a single class of sites (Bmax = 110,000/cell). The 125 I‐EGF bound was rapidly internalized at 37°C but not 4°C. Using Western blot techniques and antiphosphotyrosine antibodies, EGF induced phosphorylation of a major 170 Kd protein. Using immunoprecipitation techniques and anti‐EGF recep‐tor antibodies a major 170 Kd protein was labeled. These data indicate that biologically active EGF receptors are present on NSCLC and lung carcinoid cell lines.