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Differential growth factor expression in transformed mouse NIH‐3T3 cells
Author(s) -
Ciardiello Fortunato,
Valverius Eva M.,
ColucciD'Amato G. Luca,
Kim Nancy,
Bassin Robert H.,
S.Salomon David
Publication year - 1990
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.240420105
Subject(s) - 3t3 cells , microbiology and biotechnology , cell culture , growth factor , biology , transforming growth factor , epidermal growth factor , basic fibroblast growth factor , cell growth , fibroblast growth factor , transfection , receptor , biochemistry , genetics
The expression of growth factor‐specific mRNA transcripts and the presence of biologically active growth factors in the conditioned medium and in the cell extracts from mouse NIH‐3T3 cells transformed by different oncogences (Ki‐ ras , mos , src , fms , fes , met , and trk ), by DNA tumor virus (SV40), or by a chemical carcinogen (N‐nitrosomethylurea) were studied. In contrast to NIH‐3T3 cells or simain virus 40 (SV40)‐transformed 3T3 cells, all the other transformed NIH‐3T3 cell lines express a 4.5 kb transforming growth factor‐α (TGFα)‐specific mRNA transcript and secreted immunoreactive and biologically active TGFα ranging from 100 to 225 ng/10 8 cell/48 h. In addition, in the transformed cell lines that were secreting elevated levels of biologically active TGFα, there was a 75–95% reduction in the total number of epidermal growth factor receptors on these cells. A 2.6 kb TGFβ mRNA transcript and TGFβ protein in the conditioned medium (30–140ng/10 8 cells/48h) was also detected in those lines expressing TGFα. Basic fibroblast growth factor‐like activity (11–50 ng/10 8 cells) was detected in the cell lysates from NIH‐3T3 cells transformed with N‐nitrosomethylurea or with trk , where expression of specific 6.9 and 3.9 kb mRNA transcripts for basic fibroblast growth factor could also be found. B chain (c‐ sis) expression of platelet‐derived growth factor was present only in trk ‐transformed NIH‐3T3 cells in which specific c‐ sis 6.5 and 4.6 kb transcripts were identified. In contrast, platelet‐derived growth factor A chain expression of 2.9 and 2.3 kb transcripts was found in ras‐, met‐, mos‐, and fms ‐transformed NIH‐3T3 cells. These results suggest that the expression of different sets of growth factors is controlled in part by structurally distinct groups of transforming genes.