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Culture of primary lung tumors using medium conditioned by a lung carcinoma cell line
Author(s) -
Siegfried Jill M.
Publication year - 1989
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.240410205
Subject(s) - cell culture , lung , growth factor , in vivo , primary culture , cell growth , carcinoma , biology , secretion , chemistry , pathology , cancer research , medicine , endocrinology , biochemistry , microbiology and biotechnology , genetics , receptor
Medium conditioned by the established lung tumor cell line A549 was used as a supplement to culture cells from primary solid lung tumors. Of 36 cases placed into culture, primary cells were obtained in 33 (91.7%). Of 29 cases in which subcultures were attempted, 18 (62.1%) were successful. Nine cell lines have been established by this technique to date. In growth assays, conditioned medium (CM) was found to stimulate both monolayer colony formation and growth in semi‐solid medium of cells cultured from primary solid tumors. CM has been found to contain factors with the properties of both transforming growth factorα (TGFα) and insulin‐like growth factor‐I (IGF‐I). The addition of a combination of these factors as purified peptides to basal medium at levels found in CM (0.1–0.5 ng/ml) stimulated colony formation of lung tumor cells by up to fourfold. These results indicate that secretion of growth factors may be important in tumor growth in vivo, and that use of CM may be a valuable tool for obtaining cultures from primary solid tumors.