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Effects of bombesin on human small cell lung cancer cells: Evidence for a subset of bombesin non‐responsive cell lines
Author(s) -
KadoFong Helen,
Malfroy Bernard
Publication year - 1989
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.240400404
Subject(s) - bombesin , gastrin releasing peptide , cell culture , receptor , biology , cell , inositol phosphate , cell surface receptor , endocrinology , medicine , microbiology and biotechnology , cancer research , inositol , neuropeptide , biochemistry , genetics
The effects of bombesin on three human small cell lung carcinoma cell (SCLC) lines (NCI‐H69, NCI‐H128, and NCI‐H345) have been examined and compared to the effects of the peptide on the mouse fibroblast cell line Swiss 3T3, and the rat pituitary tumor cell line GH3W5. While all three SCLC lines expressed messenger RNA encoding pro‐gastrin releasing peptide (GRP), only the NCI‐H345 cells expressed detectable membrane receptors for GRP and responded to nanomolar concentrations of bombesin as shown by 125 I‐GRP binding, total inositol phosphate accumulation, and increased clonal growth in soft agarose. These data show that some SCLC lines are insensitive to bombesin and do not express detectable membrane receptors for GRP.