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Specific desensitization of the epidermal growth factor receptor by pp60 v‐src
Author(s) -
Gray George M.,
Wei ChungWen,
Macara Ian G.
Publication year - 1989
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.240400303
Subject(s) - cycloheximide , epidermal growth factor , proto oncogene tyrosine protein kinase src , bradykinin , receptor , microbiology and biotechnology , desensitization (medicine) , biology , heterologous , homologous desensitization , chemistry , biochemistry , protein biosynthesis , gene
BALB/c 3T3 cells infected with a temperature‐sensitive mutant (LA90) of RSV have been used to investigate possible heterologous interactions between the pp60 v‐src tyrosyl kinase and the epidermal growth factor (EGF) and bradykinin receptors. The LA90 pp60 v‐src exhibits a very rapid activation t 1/2 (<5 min) of protein kinase activity on decreasing the temperature from 40°C to 35°C. This change in temperature was also found to induce a very rapid decrease in the affinity for 125 I‐EGF of receptors on the RSV‐LA90‐infected cells but not of those on control parental cells. However, no significant changes were detected in the binding of 3 H‐bradykinin to either cell line. Two separable processes control the desensitization of the EGF receptor by pp60 v‐src , both of which are independent of protein kinase C. The first is rapid and transient, while the second is sensitive to cycloheximide and persists long after inactivation of pp60 v‐src .