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Molecular and cellular analysis of histamine H1 receptors on cultured smooth muscle cells
Author(s) -
Mitsuhashi Masato,
Payan Donald G.
Publication year - 1989
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.240400207
Subject(s) - histamine , receptor , histamine receptor , histamine h1 receptor , histamine h4 receptor , microbiology and biotechnology , stimulation , biology , histamine h2 receptor , vascular smooth muscle , cell , chemistry , endocrinology , biochemistry , smooth muscle , antagonist
Histamine is an important mediator of immediate hypersensitivity for both animals and humans. The action of histamine on target tissues is believed to be mediated by specific cell surface receptors, especially H1 and H2 receptors for hypersensitivity and inflammatory reactions, which involve stimulation of smooth muscle contractility, alterations in vascular permeability, and modifications in the activities of macrophages and lymphocytes. Although the nature of histamine receptors in the brain and peripheral tissues has been studied extensively by many laboratories, the molecular mechanism of histamine receptor‐mediated reactions is not fully understood, mainly because histamine receptors are incompletely characterized from the biochemical point of view. In previous studies, we have found that the cultured smooth muscle cell line DDT 1 MF‐2, derived from hamster vas deferens, expresses low‐affinity histamine H1 receptors and responds biochemically and functionally to H1‐specific stimulation (Mitsuhashi and Payan, J Cell Physiol 134:367, 1988). This cell line provides a model for analyzing the biochemical responses of H1 receptor‐mediated reactions in peripheral tissues. In this review, we summarized our recent progress in the study of low‐affinity H1 receptors on DDT 1 MF‐2 cells.