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Transforming growth factor‐β does not alter interleukin‐1 expression in cultured human macrophages
Author(s) -
Hurme Mikko,
Sihvola Marja,
Alitalo Kari,
KeskiOja Jorma
Publication year - 1989
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.240390412
Subject(s) - transforming growth factor , lipopolysaccharide , plasminogen activator , interleukin , biology , growth factor , microbiology and biotechnology , cell culture , extracellular , chemistry , cytokine , endocrinology , immunology , biochemistry , receptor , genetics
Transforming growth factor‐β (TGFβ) is a growth modulator that stimulates the growth of fibroblastic cells but inhibits the growth of cells of epithelial origin. TGFβ also influences the production of extracellular matrix proteins, and of proteases and the type 1 plasminogen activator inhibitor (PAI‐1) by cultured cells. TGFβ appears also to have various immunoregulatory effects, suppressing both T‐ and B‐cell activities. It has been proposed that it might increase the expression of interleukin‐1 (IL‐1) mRNA in cultured human monocytes, thus potentiating immune functions. To analyze the role of TGFβ in IL‐1 production we have now quantitated the effect of this factor on the production of biologically active IL‐1 as well as IL‐1β mRNA expression. The effect of TGFβ on IL‐1 production optimally activated with bacterial lipopolysaccharide (LPS) was also studied. It was found that IL‐1 activity and mRNA levels were rapidly elevated by LPS but not by TGFβ. Culture fluids from monocytes treated with TGFβ alone or with TGFβ plus LPS inhibited the proliferation of the test thymocytes. After gel filtration, the media from TGFβ‐treated cultures showed no activity in the molecular weight area of IL‐1 (approx. 15 kD), while the supernatants from TGFβ plus LPS‐induced cells contained IL‐1 activity in these fractions, the magnitude of which was, however, at the same level as in the culture fluids derived from cells stimulated with LPS alone. Thus our results show that the TGFβ used was biologically active but they provide no evidence for TGFβ in the regulation of IL‐1 production in human monocytes.