Premium
Type I protein kinase C isozyme in the visual‐information‐processing pathway of monkey brain
Author(s) -
Huang Freesia L.,
Yoshida Yasuyoshi,
Nakabayashi Hiroki,
Friedman David P.,
Ungerleider Leslie G.,
Young W. Scott,
Huang KuoPing
Publication year - 1989
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.240390406
Subject(s) - hippocampal formation , neuroscience , cerebellum , amygdala , hippocampus , neocortex , limbic lobe , protein kinase c , cerebral cortex , biology , dentate gyrus , temporal lobe , temporal cortex , chemistry , microbiology and biotechnology , kinase , epilepsy
Previously using PKC isozyme‐specific antibodies for immunoblot analysis, we demonstrated the heterogeneous distribution of PKC isozymes in various regions of monkey and rat brains and that type I PKC was most abundant in cerebellum, hippocampus, amygdala, and cerebral cortex (Huang et al.: J Biol Chem 262:15714–15720, 1987). Using these antibodies, we have also demonstrated that type I, II, and III PKC are products of PKC genes γ, β, and α, respectively (Huang et al.: Biochem Biophys Res Commun 149:946–952, 1987). By immunocytochemical analysis, type I PKC‐specific antibody showed strong reactivity in various types of neuron in hippocampal formation, amygdala, cerebellum, and neocortex. In hippocampal formation, granule cells of dentate gyrus and pyramidal cells of hippocampus were heavily stained. By immunoblot analysis, relative levels of PKC isozymes in several areas of monkey cerebral cortex involved in the visual information processing and storage were determined. Both type II and III PKCs appeared to be evenly distributed and at moderate levels, type I PKC formed a gradient of increasing concentration rostral along the cerebral cortex of occipital to temporal and then to the limbic areas. Neurobehavioral studies have demonstrated that the neocortical and limbic areas of the anterior and medial temporal regions participate more directly than the striate, prestriate, and posterior temporal regions in the storage of visual representations and that both hippocampus and amygdala are important in the memory formation. As type I PKC is present at high levels in hippocampus, amygdala, and anterior temporal lobe, we predict that the type I protein kinase C may participate in the plastic changes important for mnemonic function.