Premium
A polypeptide growth inhibitor isolated from lactating bovine mammary gland (MDGI) is a lipid‐carrying protein
Author(s) -
Böhmer FrankD.,
Mieth Maren,
Reichmann Gunter,
Taube Christel,
Grosse Richard,
Hollenberg Morley D.
Publication year - 1988
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.240380307
Subject(s) - palmitic acid , fatty acid , biochemistry , biology , fatty acid binding protein , in vitro , function (biology) , peptide sequence , endogeny , microbiology and biotechnology , gene
Mammary‐derived growth inhibitor (MDGI), a polypeptide growth inhibitor isolated from lactating bovine mammary tissue, previously shown to have extensive sequence homology with fatty acid‐binding proteins, was demonstrated to meet the criteria of a fatty acid‐binding protein. The protein was found to bind [ 3 H]palmitic acid in a saturable manner and to be complexed with endogeneous free fatty acids. [ 3 H]palmitic acid, when bound to the protein, was more rapidly taken up by the target cells (human mammary carcinoma cells [MaTu]) than was free [ 3 H]palmitic acid, suggesting a lipid carrier function for the inhibitor. It is suggested that the fatty acid‐binding properties of MDGI may relate to its ability to inhibit cell growth in vitro and to regulate other cellular functions.