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Cation‐independent mannose 6‐phosphate receptor contains covalently bound fatty acid
Author(s) -
Westcott Keith R.,
Rome Leonard H.
Publication year - 1988
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.240380104
Subject(s) - palmitic acid , chemistry , mannose , hydroxylamine , mannose 6 phosphate , biochemistry , myristic acid , receptor , fatty acid , phosphate , free fatty acid receptor , polyunsaturated fatty acid , growth factor
The cation‐independent mannose 6‐phosphate receptor (215,000 daltons) was isolated from embryonic bovine tracheal cells and embryonic human skin fibroblasts labelled with [ 3 H]palmitic acid, the tritium label was detected in the protein upon fluorographic analysis of SDS‐polyacrylamide gels of the purified receptor. The label was not sensitive to hydroxylamine, methanolic KOH, or β‐mercaptoethanol, but labelled fatty acid was recovered from the protein by acidic methanolysis. Labelled receptor protein could not be isolated from cells grown in the presence of [ 3 H]myristic acid. The results suggest the presence of amide‐linked palmitic acid in the structure of the cation‐independent mannose 6‐phosphate receptor.