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Inhibition of metastatic potential by fucosidase: An NMR study identifies a cell surface metastasis marker
Author(s) -
Wright Lesley C.,
May George L.,
Gregory Patricia,
Dyne Marlen,
Holmes Kerry T.,
Williams Philip G.,
Mountford Carolyn E.
Publication year - 1988
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.240370106
Subject(s) - malignancy , chemistry , nuclear magnetic resonance spectroscopy , metastasis , cell , fucosidase , cancer research , t2 relaxation , magnetic resonance imaging , fucose , nuclear magnetic resonance , pathology , medicine , cancer , biochemistry , stereochemistry , physics , galactose , radiology
NMR spectroscopy is able to detect subtle changes to the surface chemistry of cells. We have previously shown that high‐resolution 1 H NMR methods can identify tumor cells with the capacity to metastasize, and we now report that the long T 2 relaxation value (500–800 ms) observed in metastatic rat mammary adenocarcinoma cells is removed by treatment with fucosidase. Two‐dimensional scalar‐correlated NMR (COSY) spectra of fucosidase‐treated cells show that a cross peak, consistent with scalar coupling between the methyl and methinè groups on fucose and usually associated with malignancy and metastatic ability, is absent. Metastases were observed in only two out of ten rats injected subcutaneously with enzyme‐treated cells compared to eight out of ten with untreated cells. NMR studies on isolated cellular lipids identified the long T 2 relaxation value only in the ganglioside fraction. This fraction accounts for 51% of the total 14 C‐labelled fucose incorporated into the cells. We propose that fucogangliosides are an indicator of metastatic potential in rats. The observation that a cell surface metastasis marker has an NMR signal with a characteristically long relaxation value has important consequences for the future use of magnetic resonance imaging and spectroscopy in the cancer clinic.