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Swiss 3T3 mouse embryo fibroblasts transfected with a human prepro‐GRP gene synthesize and secrete pro‐GRP rather than GRP
Author(s) -
LebacqVerheyden AnneMarie,
Segal Shoshana,
Cuttitta Frank,
Battey James F.
Publication year - 1988
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.240360305
Subject(s) - transfection , embryo , 3t3 cells , secretion , biology , microbiology and biotechnology , gene , genetics , biochemistry
A prepro‐gastrin‐releasing peptide (GRP) gene was introduced into Swiss 3T3 mouse embryo fibroblasts by DNA transfection in an attempt to establish autocrine growth stimulation. Clonal transfectants expressed varying amounts of GRP encoding mRNA. They synthesized and secreted a ∼ 15‐kd pro‐GRP hormone but not fully processed 2.8‐kd GRP. Accordingly, no changes in growth properties were associated with GRP gene expression. We postulate that Swiss 3T3 fibroblasts lack the enzymes necessary to process significantly pro‐GRP into biologically active peptides and that this deficiency may be responsible for the failure to establish autocrine growth stimulation in the transfected cells.