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Spontaneous fusion between metastatic mammary tumor subpopulations
Author(s) -
Miller Fred R.,
McInerney Donna,
Rogers Clare,
Miller Bonnie E.
Publication year - 1988
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.240360204
Subject(s) - ouabain , aminopterin , cell culture , hypoxanthine , cell fusion , in vitro , cancer research , thymidine , metastatic breast cancer , microbiology and biotechnology , chemistry , biology , medicine , biochemistry , immunology , cancer , breast cancer , genetics , methotrexate , organic chemistry , enzyme , sodium
This study describes a differential frequency of spontaneous fusion between metastatic and nonmetastatic subpopulations derived from a single mouse mammary tumor. Subpopulations 66, 66cl4 (a variant of 66 which is resistant to both thioguanine and ouabain), 410.4, and 44FTO (a thioguanine‐resistant, ouabain‐resistant derivative of 410.4) spontaneously metastasize from subcutaneous and mammary fatpad sites. Subpopulations 168, 168FARO (a diaminopurine‐resistant, ouabain‐resistant derivative of 168), 67, 68H, and 410 do not. The ability of these subpopulation lines to fuse spontaneously in vitro was determined after coculturing a drug‐resistant line with a wild‐type line in nonselective media. After 16–20 h of coculture, cells were plated in the appropriate media to select for fusion products—either HAT (hypoxanthine, aminopterin, thymidine) plus ouabain or AA (alanosine, adenine) plus ouabain—to determine the number of colony‐forming cells (fusion products) present per 10 4 cells plated. When both subpopulations of the pair in the fusion mixture were metastatic, a significantly greater number of fusion products was recovered than if one or both of the subpopulations in the fusion mixture was nonmetastatic, with one exception: line 410 readily fused with both 66cl4 and 44FTO. Subline 410 was highly metastatic when originally isolated but lost its metastatic competence after a brief time in tissue culture.