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Crosslinking of DNA to nuclear lamina proteins by UV irradiation in vivo
Author(s) -
GalchevaGargova Z.,
Dessev G. N.
Publication year - 1987
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.240340303
Subject(s) - lamin , nuclear lamina , dna , covalent bond , in vivo , irradiation , chemistry , vimentin , biophysics , microbiology and biotechnology , biochemistry , nuclear protein , biology , gene , immunohistochemistry , genetics , physics , organic chemistry , transcription factor , nuclear physics , immunology
We have been able to demonstrate that a fraction of DNA becomes crosslinked to nuclear lamina shells isolated from Ehrlich ascites tumour cells irradiated with UV light. Terminal labeling of short DNA fragments covalently attached to proteins reveals that DNA has become crosslinked to all three lamins and to a protein comigrating with vimentin.