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Differential induction of transcription of c‐ myc and c‐ fos proto‐oncogenes by 12‐O‐tetradecanoylphorbol‐13‐acetate in mortal and immortal human‐urothelial cells
Author(s) -
Skouv Jan,
Christensen Britta,
Autrup Herman
Publication year - 1987
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.240340202
Subject(s) - 12 o tetradecanoylphorbol 13 acetate , cell culture , transcription (linguistics) , microbiology and biotechnology , transcription factor , cell , biology , tetradecanoylphorbol acetate , chemistry , cancer research , gene , phorbol ester , signal transduction , biochemistry , genetics , protein kinase c , linguistics , philosophy
The effect of the skin tumor‐promoter TPA (12‐O‐tetradecanoylphorbol‐13‐ace‐tate) on expression of cellular proto‐oncogenes has been examined in cell lines derived from human urothelium. A single treatment with TPA (1 μg/ml) increased the transcription of c‐ fos and c‐ myc proto‐oncogenes at least 20‐fold in the mortal cell line HU 1752. The induction was transient and was accompanied by a rapid but transient change in cell morphology. When immortalized cell lines were treated with TPA a similar rapid and transient morphological response was observed, but the TPA treatment only increased the level of c‐ fos mRNA. suggesting that the normal regulation of c‐ myc transcription is altered in immortalized cells irrespective of their tumorigenic properties. The levels of c‐Ha‐ ras and c‐Ki‐ ras mRNAs were unaffected by TPA treatment in all cell lines.