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Suppression of tumorigenicity in somatic cell hybrids does not involve quantitative changes in transcription of cellular Ha‐ras, Ki‐ras, myc, and fos oncogenes
Author(s) -
Schäfer R.,
Geisse S.,
Willecke K.
Publication year - 1987
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.240340105
Subject(s) - biology , chinese hamster , somatic cell , phenotype , microbiology and biotechnology , gene , hybrid , cell fusion , somatic fusion , transcription factor , cell growth , hamster , cell , cell culture , genetics , botany
The transcriptional activity of ten cellular oncogenes was analyzed in somatic cell hybrids that had been obtained after fusion of tumorigenic Chinese hamster cells and normal mouse fibroblasts. The hybrids showed either the tumorigenic or the nontumorigenic phenotype (suppression of tumorigenicity). Out of ten c‐one genes analyzed, four (c‐Ha‐ras, c‐Ki‐ras, c‐myc, and c‐fos) were found to be transcriptionally active at similar levels in tumorigenic as well as in nontumorigenic (suppressed) hybrids. Thus we conclude that suppression of tumorigenicity in Chinese hamster × mouse somatic cell hybrids does not correlate with quantitative changes in expression of these cellular oncogenes. The remaining six cellular oncogenes (c‐abl, c‐erb A and B, c‐fes, c‐myb, and c‐sis) were not transcriptionally active in these hybrids.