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A monoclonal antibody reactive with an activated ras protein expressing valine at position 12
Author(s) -
Carney W. P.,
Hamer P.,
Petit D.,
Rabin H.,
Cooper G.,
Lefebvre M.,
Wolfe H.
Publication year - 1986
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.240320307
Subject(s) - serine , microbiology and biotechnology , valine , monoclonal antibody , alanine , amino acid , cysteine , arginine , aspartic acid , biology , biochemistry , glycine , antibody , cell culture , chemistry , phosphorylation , enzyme , genetics , immunology
Activated ras transforming genes have been described in a variety of neoplasms and encode 21,000‐Dalton (p21) proteins with amino acid substitutions at positions 12, 13, and 61. In this report we describe a monoclonal antibody designated DWP that reacts. Specifically with synthetic dodecapeptides containing valine at position 12, to a lesser extent with peptides containing cysteine at position 12 and not with peptides containing glycine, arginine, serine, aspartic acid, glutamic acid or alanine at the same position. Western blot and immunoperoxidase studies showed that DWP specifically reacts with activated ras H or ras K proteins in NIH cells transformed by DNA from the human carcinoma cells that encode valine at position 12. DWP did not react with normal p21s encoding glycine at position 12, nor with activated p21s encoding aspartic acid, glutamic acid, arginine, serine, or cysteine at position 12. A survey of human tumor cell lines demonstrated that DWP reacted with the human bladder carcinoma cell line T24 but not with human tumor cell lines previously shown td contain other activating mutations at positions 12 or 61. DWP and perhaps additional antibodies that specifically react with alterations at positions 12 or 61 of the ras protein may be valuable in determining the presence and frequency of activated ras proteins in human malignancy.