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Antipeptide antibodies directed against the carboxy‐terminal region of SV40 structural proteins VP2 and VP3
Author(s) -
Streckert HansJürgen,
Brüssow Harald,
Sure Klaus,
Werchau Hermann
Publication year - 1986
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.240310405
Subject(s) - terminal (telecommunication) , antibody , chemistry , microbiology and biotechnology , biology , biochemistry , immunology , computer science , telecommunications
Rabbits were immunized with a synthetic heptapeptide of the sequence Arg‐Asn‐Arg‐Ser‐Ser‐Arg‐Ser corresponding to the carboxy‐terminal region of the SV40 viral proteins VP2 and VP3. The raised antibodies recognize the viral proteins in enzyme‐linked immunosorbent (ELISA) and Western blot assay. Specificity of the antibodies were confirmed by competition experiments. The antibodies recognize VP2 and VP3 in infected cells by immunofluorescence and in subcellular fractions by ELISA. No interaction with virions was observed.