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Identification of a biochemical marker for the secretory pathway in Tetrahymena thermophila
Author(s) -
Maihle Nita J.,
Satir Birgit H.
Publication year - 1986
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.240310302
Subject(s) - tetrahymena , biogenesis , secretory protein , antiserum , biology , secretion , microbiology and biotechnology , immunofluorescence , secretory pathway , identification (biology) , biochemistry , antigen , antibody , genetics , golgi apparatus , gene , endoplasmic reticulum , ecology
Tetrahymena thermophila is a ciliated protozoan studied by investigators from a wide range of disciplines as a model system for a variety of specialized eukaryoticcell functions. The proteinaceous secretory products of T thermophila have been isolated and characterized [1] and in this study we identify the major secretory product, a 34,000 Mr polypeptide, and use an antiserum prepared against this secretory protein to (1) demonstrate that this 34,000 Mr polypeptide is truly a secreted protein in Tetrahymena and (2) monitor the synthesis and transport of this protein by indirect immunofluorescence and light microscopy during mucocyst biogenesis.