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Immunoreactive fibroblast growth factor (FGF) in a transplantable chondrosarcoma: Inhibition of tumor growth by antibodies to FGF
Author(s) -
Baird Andrew,
Mormède Pierre,
Böhlen Peter
Publication year - 1986
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.240300109
Subject(s) - radioimmunoassay , fibroblast growth factor , antiserum , antibody , chemistry , endocrinology , fibroblast , microbiology and biotechnology , medicine , biology , cancer research , biochemistry , immunology , receptor , in vitro
Using a radioimmunoassay for bovine pituitary fibroblast growth factor (FGF), we have established the presence of the immunoreactive mitogen in extracts of a transplantable mouse chondrosarcoma. Both neutral and acidic extracts of the tumor contain an immunoreactive FGF (ir‐FGF) that cross‐reacts in a parallel and dose‐dependent fashion in the radioimmunoassay. The ir‐FGF is retained on heparin‐Sepharose affinity columns and can be detected in the same molecular weight forms as rat pituitary FGF. Mice (C57/B1) inoculated with the tumor (10 mg, im) show a decreased rate of tumor growth when passively immunized with the antiserum to FGF. The results establish the presence of FGF in this tumor and implicate its role in the etiology of its development.