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12‐O‐Tetradecanoylphorbol‐13‐acetate (TPA)‐induced gene sequences in human primary diploid fibroblasts and their expression in SV40‐transformed fibroblasts
Author(s) -
Angel Peter,
Rahmsdorf Hans Jobst,
Pöting Annette,
LückeHuhle Christine,
Herrlich Peter
Publication year - 1985
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.240290408
Subject(s) - 12 o tetradecanoylphorbol 13 acetate , gene , microbiology and biotechnology , mitomycin c , rna , ploidy , complementary dna , fibroblast , biology , gene expression , cell culture , chemistry , biochemistry , genetics , signal transduction , protein kinase c , phorbol ester
We have isolated cDNA sequences from TPA‐treated primary human fibroblasts, which indicate RNA species that are coordinately regulated after treatment of these cells with either ultraviolet light, mitomycin C, the UV‐induced factor EPIF, or TPA. The levels of RNA are elevated in Bloom syndrome (cells of two out of three patients). After transformation with SV40 one of the sequences is overexpressed while another one is reduced. Both genes maintain their inducibility by the agents mentioned.