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Angiogenesis is stimulated by a tumor‐derived endothelial cell growth factor
Author(s) -
Shing Yuen,
Folkman Judah,
Haudenschild Christian,
Lund Dennis,
Crum Rosa,
Klagsbrun Michael
Publication year - 1985
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.240290402
Subject(s) - angiogenesis , chorioallantoic membrane , growth factor , 3t3 cells , endothelial stem cell , microbiology and biotechnology , biology , affinity chromatography , cornea , cell growth , chemistry , cell culture , biochemistry , in vitro , cancer research , transfection , receptor , genetics , neuroscience , enzyme
A growth factor mitogenic for BALB/C 3T3 cells and capillary endothelial cells was isolated from a rat chondrosarcoma and purified to homogeneity. Purification was accomplished by a combination of BioRex 70 cation exchange chromatography and heparin affinity chromatography. The pure chondrosarcoma‐derived growth factor (ChDGF) had a molecular weight of about 18.000. The angiogenesis activity of pure ChDGF was tested by measuring its ability to vascularize the chorioallantoic membrane (CAM) and yolk sac membrane of the developing chick. The ability of ChDGF to induce the growth of limbal vessels in the rat cornea was also measured. To quantitate the angiogenesis response, a unit system based on the growth factor activity of ChDGF for 3T3 cells was adopted. ChDGF was found to have a specific activity of about 5 units/ng when applied to 3T3 cells. About 300–600 units of ChDGF in the two types of developing chick membrane and 30–50 units of ChDGF in the rat cornea were found to stimulate noninflammatory angiogenesis.