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Structure and chromosomal localization of the human lymphotoxin gene
Author(s) -
Nedwin Glenn E.,
JarrettNedwin Julie,
Smith Douglas H.,
Naylor Susan L.,
Sakaguchi Alan Y.,
Goeddel David V.,
Gray Patrick W.
Publication year - 1985
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.240290302
Subject(s) - gene , biology , lymphotoxin , microbiology and biotechnology , genetics , homology (biology) , southern blot , promoter , dna , genomic dna , chromosome , genomic library , peptide sequence , gene expression , immune system
We have isolated, sequenced, and determined the chromosomal localization of the gene encoding human lymphotoxin (LT). The single copy gene was isolated from a human genomic library using a 32 P‐labeled 116 bp synthetic DNA fragment whose sequence was based on the NH 2 ‐terminal amino acid sequence of LT. The gene spans 3 kb of DNA and is interrupted by three intervening sequences. The LT gene is located on human chromosome 6, as determined by Southern blot analysis of human‐murine hybrid DNA. Putative transcriptional control regions and areas of homology with the promoters of interferon and other genes are identified.