Premium
Inhibition of ornithine decarboxylase activity and cell growth by diamines: A comparison between the effects of two homologs, 1,3‐diaminopropane and 1,4‐diaminobutane (putrescine)
Author(s) -
Linden Margareta,
Oredsson Stina M.,
Anehus Siw,
Heby Olle
Publication year - 1985
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.240290206
Subject(s) - putrescine , spermidine , spermine , ornithine decarboxylase , polyamine , biochemistry , chemistry , ornithine , enzyme , biology , microbiology and biotechnology , arginine , amino acid
The ornithine decarboxylase (ODC) activity of Ehrlich ascites tumor cells was almost completely inhibited by treatment with either putrescine (10 mM) or 1,3‐diaminopropane (5 mM). 1,3‐Diaminopropane treatment eradicated the cellular content of putrescine and reduced that of spermidine and spermine. Putrescine treatment caused a dramatic increase in cellular putrescine content and a temporary decrease in spermidine and spermine content. Despite the fact that 1,3‐diamino‐propane and putrescine inhibited the ODC activity more effectively than did α‐difluoromethylornithine (DFMO), an enzyme‐activated irreversible inhibitor of ODC, they were considerably less antiproliferative in action. However, as compared to DFMO the diamines were less effective in reducing the total polyamine (putrescine + spermidine + spermine) content of the cells.