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Phenazine methosulfate stimulation of ouabain‐sensitive Rb + uptake by HeLa cells: Effects of respiratory inhibitors, anaerobiosis, and ascorbate
Author(s) -
Ikehara Toshitaka,
Yamaguchi Hisao,
Hosokawa Keiko,
Kaku Masayuki,
Miyamoto Hiroshi
Publication year - 1985
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.240280405
Subject(s) - stimulation , ouabain , hela , chemistry , respiration , rotenone , intracellular , respiratory system , respiratory chain , biochemistry , enzyme , medicine , endocrinology , sodium , biology , cell , mitochondrion , botany , organic chemistry
Phenazine methosulfate (PMS) stimulates ouabain‐sensitive Rb + uptake by HeLa cells. This stimulation cannot be attributed to the effect of the dye on the intracellular Na + or ATP content. Respiratory inhibitors, such as 5 mM NaCN and 5 mM rotenone, and anaerobic conditions enhance the stimulation of Rb + uptake by PMS. Cellular respiration is stimulated, but lactate production is reduced in the presence of PMS, irrespective of the presence of respiratory inhibitors. Cellular NADH is oxidized markedly on addition of PMS plus inhibitors, but it is not affected by addition of the inhibitors only. In the presence of a high concentration of PMS, PMS‐stimulated ouabain‐sensitive Rb + uptake is inhibited by addition of ascorbate. From these results it is concluded that Na/K‐pump activity is closely related to the cellular redox state.