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Evidence for p15 cleavage site in myc‐specific proteins of avian MC29 and OK10 viruses
Author(s) -
Sovová Vlasta,
Trávníček Miloslav,
Hložánek Ivo,
Černá Hana,
Alitalo Kari,
Vaheri Antti
Publication year - 1985
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.240280404
Subject(s) - cleavage (geology) , virus , virology , biology , microbiology and biotechnology , fracture (geology) , paleontology
Myc‐related proteins were precipitated from MC29 virus‐transformed cells (PR‐2) and from OK10 virus‐transformed cells (9C) by anti‐gag and anti‐myc sera. Immunoprecipitates were cleaved with the avian retroviral protease p15 and the cleavage products analyzed in SDS‐PAGE. Cleavage fragments of p110 gag‐myc (product of MC29 virus) and p58 myc (product of OK10 virus) showed the presence of a p15 cleavage site within the myc‐specific region. The site is missing in deletion mutants of MC29 virus.