Premium
Matrix influence on the tumor cell stimulation of fibroblast collagenase production
Author(s) -
Biswas Chitra
Publication year - 1985
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.240280107
Subject(s) - collagenase , fibroblast , matrix (chemical analysis) , stimulation , chemistry , microbiology and biotechnology , cell culture , extracellular matrix , cell , in vitro , biology , endocrinology , biochemistry , enzyme , genetics , chromatography
Cocultures of rabbit fibroblasts and mouse B‐16 melanoma cells produce increased levels of collagenase against type I collagen. This stimulatory effect was also found when fibroblasts were cultured in conditioned media from tumor cells. However, the level of the stimulatory factor in conditioned media was influenced by matrix deposited by fibroblasts. Thus, conditioned media collected from mono‐layers of B‐16 plated on fibroblast matrix consistently showed high levels of the factor activity. The influence of the matrix on the level of the factor was not removed by treating the fibroblast matrix with collagenase or chondroitinase ABC and was not reproduced by collagen‐coated dishes.